Abstract: ObjectiveTo clone and express VP1-VP4genes encoding the structural proteins of Coxsackie virus A16and analyze
the antigenicity of the expressed recombinant proteins.MethodsThe VP1-VP4cDNAs were amplified with RT-PCR from the
extracted viral RNA and cloned into pMD19-T vectors. The VP1-VP4genes were inserted to the multi-cloning sites of the
plasmid pQE30a, and the protein expressions inE. coliM15were induced by IPTG. After purification by washing with8mol/L
urea under denaturing condition, the recombinant proteins were identified by Western blotting and ELISA for their
immunogenicity against rabbit antisera of Coxsackie virus A16and enterovirus71, respectively. ResultsThe recombinant
VP1-VP4proteins were highly expressed inE. coliM15. The purified proteins could be recognized by rabbit antiserum of
Coxsackie virus A16and showed cross reactivity with the rabbit antiserum of Enterovirus71. ConclusionThe recombinant
Coxsackie virus A16VP1-VP4proteins obtained possess good antigenicity.