Abstract: ObjectiveTo investigate the changes in the microRNA expression profile of Vero cells induced by HSV-2LAT
overexpression.MethodsThe full-length open reading frame of HSV-2 LAT was synthesized and cloned into pRetroQ-AcGFP1-C1vector, and the recombinant retrovirus expressing HSV-2LAT was packaged. Using a microRNA microarray, the
microRNA expression profile changes in Vero cells were analyzed after infection with the recombinant retrovirus.ResultsIn
Vero cells infected with the recombinant retrovirus for stable HSV-2 LAT overexpression,5 microRNAs (hsa-miR-23a*,
kshv-miR-K12-3, hsa-miR-943, hsa-miR-634, and hsa-miR-1270) were up-regulated and 5(hsa-miR-181a-2*, hsa-miR-450b-5p,
hsa-miR-31, hsa-miR-24, and kshv-miR-K12-12*) were down-regulated.ConclusionThe expression of HSV-2LAT can induce
changes in microRNA expression profile in Vero cells.