Journal of Southern Medical University

Journal of Southern Medical University ›› 2006, Vol. 26 ›› Issue (11): 1574-1576.

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Establishment of miniCTTA multiplex set with fluorescence-labeled primers

LIU Chao1,2,WANG Hui-pin2,3,SUN Hong-yu3,WANG Hui-jun1 1Institute of Forensic Medicine,Southern Medical University,Guangzhou 510515,China;2Guangzhou Criminal Science and Technology Institute,Guangzhou 510030,China;3Department of Forensic Biology,Sun Yat-sen University,Guangzhou 510080,China   

  1. 南方医科大学法医学研究所； 广州市刑事科学技术研究所； 中山大学法医学系； 南方医科大学法医学研究所 广东广州510515广州市刑事科学技术研究所； 广东广州510030； 广东广州510030中山大学法医学系； 广东广州510080； 广东广州510515；
  • Online:2006-11-20 Published:2006-11-20

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Abstract: Objective To establish a miniCTTA multiplex set including short tandem repeat(STR) markers as CSF1PO,TH01,and TPOX and amelogenin gene,whose amplified fragments are shorter than 130 bp.Methods The length of the gene fragments amplified with fluorescence-labeled primers were analyzed by ABI3100 Genetic Analyzer for genotyping of 100 unrelated individuals,10 genealogies and 30 highly degraded specimens.Results The genotypes derived form miniCTTA were consistent with the results by AmpFLSTR Identifiler kit.Conclusion MiniCTTA multiplex set is useful for personal identification and paternity test,especially applicable for degraded DNA sample. 

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