Journal of Southern Medical University ›› 2006, Vol. 26 ›› Issue (10): 1383-1387.

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Fenofibrate inhibits tumor necrosis factor-α-induced expression of CD40 and matrix metalloproteinase in human vascular endothelial cells

LIN Rong1, 2, LIU Jun-tian1, GAN Wei-jie1, WANG Wei-rong1, HAN Chun-jie1, LIU Yu 1, FANG Zhi-yuan1 1Department of Pharmacology, 2Key Laboratories of Environment and Genes Related Diseases of Ministry of Education, Xi’an Jiaotong University, Xi’an 710061, China   

  1. 西安交通大学医学院药理学教研室; 西安交通大学医学院药理学教研室 西安交通大学环境与疾病相关基因教育部重点实验室; 陕西西安710061;
  • Online:2006-10-20 Published:2006-10-20

Abstract: Objective To investigate the regulatory effects of fenofibrate on TNF-α-induced CD40 expression and matrix metalloproteinase (MMP) activity in human vascular endothelial cells (HUVECs). Methods Quantitative RT-PCR and flow cytometry were employed to evaluate the effect of fenofibrate on TNF-α-induced CD40 mRNA and cell surface CD40 expression in HUVECs, and gelatin zymography was used to determine the effect of fenofibrate on the gelatinolytic activities of MMP-2 and MMP-9 in TNF-α-stimulated HUVECs. Results Fenofibrate at the concentrations of 5×10-5, 1×10-4 and 2×10-4 mol/L significantly reduced TNF-α-induced increment of CD40 mRNA and cell surface CD40 expressions (P<0.01), with the maximal inhibition achieved at the concentration of 1×10-4 mol/L. Fenofibrate at 2×10-4 mol/L did not further decrease CD40 expression induced by TNF-α. Fenofibrate significantly inhibited the stimulatory effect of TNF-α on MMP-2 and MMP-9 activities in HUVECs. Conclusion Fenofibrate reduces TNF-α-induced increment of CD40 expression and MMP-2 and MMP-9 activities in HUVECs. 

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