Abstract: Objective To evaluate the clinical outcomes of allogeneic bone grafting for bone defect resulting from benign neoplasm resection and discuss the clinical application and bone defect repair mechanisms of allogeneic bone. Methods A retrospective review was conducted of 135 patients with benign neoplasm resection who received bone defect filling with the allogeneic bone graft. Results In the 104 patients with complete clinical follow-up data, 96 achieved bone union, 7 experienced relapses to require surgical intervention and 1 had severe infection to lead to failure of the operation. The mean time for bone union was 9.7 months, and during the follow-up, no viral disease in relation to the graft was found after surgery. Conclusion Bone defect filling with allogeneic bone graft can be simple and safe in comparison with that with autograft or other biomaterials, and the bone healing time, infection rate and local tumor recurrence can be comparable with the autograft.