Recombinant human endostatin induces apoptosis of human umbilical venous endothelial cells

Abstract

Abstract: To investigate the effects of human endostatin on proliferation of human umbilical venous endothelial cells, the apoptosis of the endothelial cells treated with recombinant human endostatin was examined by fluorescence method and the cell proliferation activity analyzed by MTT assay. The results showed that human endostatin inhibited the proliferation of the endothelial cells with ED50 of 550 ng/ml, and the inhibitory effect was enhanced with the increase of endostatin concentration, suggesting a dose-dependent inhibitory effect of human endostatin on endothelial cell proliferation. The mechanism of antiangiogenesis induced by human endostatin is related to the inhibition of cell proliferation and induction of apoptosis of the endothelial cells.

CLC Number:

YANG Fang, HE Yuan-li Department of Obstetrics and Gynecology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Recombinant human endostatin induces apoptosis of human umbilical venous endothelial cells[J]. Journal of Southern Medical University, 2006, 26(07): 978-980.

[1]	LI Jin-long, CAI Yu-chen, , HU Zhi-ming, GAO Ji-min . Norcantharidin inhibits DNA replication initiation protein Cdc6 in cancer cells [J]. Journal of Southern Medical University, 2010, 30(08): 1851-1853.
[2]	YANG Ling,CHEN Zhi-liang,LIU Shi-ting,YANG Li Department of Pharmacy,Nanfang Hospital,Southern Medical University,Guangzhou 510515,China. Sampling of exhaled gas after intravenous administration of octafluoropropane-containing human albumin micropheres in dogs [J]. Journal of Southern Medical University, 2006, 26(12): 1706-1708.
[3]	WU Wen1,DING He-lin2,CHEN Li-hong2,FU Zu-zhi2 1Department of Endocrinology,Institute of Geriatrics,People’s Hospital of Guangdong Province,Guangzhou 510080,China;2Department of Endocrinology,Second Hospital of Sun Yat-sen University,Guangzhou 510120,China. Effects of losartan on the levels of angiotensinⅡ and its type-1 receptor in diabetic rat kidney [J]. Journal of Southern Medical University, 2006, 26(12): 1742-1744.
[4]	LIN Rong1, 2, LIU Jun-tian1, GAN Wei-jie1, WANG Wei-rong1, HAN Chun-jie1, LIU Yu 1, FANG Zhi-yuan1 1Department of Pharmacology, 2Key Laboratories of Environment and Genes Related Diseases of Ministry of Education, Xi’an Jiaotong University, Xi’an 710061, China. Fenofibrate inhibits tumor necrosis factor-α-induced expression of CD40 and matrix metalloproteinase in human vascular endothelial cells [J]. Journal of Southern Medical University, 2006, 26(10): 1383-1387.
[5]	FAN Ying-ying1, XU Li-xian1, WEN Ai-dong2, ZHANG Hui1, LIU Chun-ran1, LI Wei2, MEI Xiao-peng1 Department of Anesthesiology, Stomatology Hospital1, Department of Pharmacy, Xijing Hospital2, Fourth Military Medical University, Xi’an 710033, China. Determination of propofol in human serum by improved reversed phase high-performance liquid chromatography with fluorescence detection [J]. Journal of Southern Medical University, 2006, 26(10): 1510-1512.
[6]	CHEN Juan, ZOU Zhi-peng, WU Zhong-Hai Department of Physiology, Southern Medical University, Guangzhou 510515, China. Effects of pentobarbital sodium on rhythmical respiration of neonatal rat medullary preparations [J]. Journal of Southern Medical University, 2006, 26(09): 1273-.
[7]	ZHU-jun1, YANG-hong1, LIU Wei-zhong2 1Department of Pharmacy, Second Affiliated Hospital, Sun Yat-sen University, Guangzhou 510120, China; 2Base of Clinical Drug Study, Guangzhou Psychiatry Hospital, Guangzhou 510370, China. Reversed phase high-performance liquid chromatography for determination of plasma olanzapine and pharmacokinetics of olanzapine in healthy Chinese volunteers [J]. Journal of Southern Medical University, 2006, 26(09): 1327-1329.
[8]	LU Jin-li1, YANG Fang2 Department of Radiotherapy, First Hospital1, Western District Library2, Xi’an Jiaotong University, Xi’ an 710061, China. Clinical pharmacokinetic study of cisplatin in enhancing cervical cancer radiosensitivity [J]. Journal of Southern Medical University, 2006, 26(08): 1170-1172.
[9]	MA Wei, WANG Zuo-ren, ZHANG Yun-feng, SHI Lei Department of Hepatobiliary Surgery, First Hospital of Xi’an Jiaotong University, Xi’an 710061, China. Effect of prostaglandin E1 on monocyte chemotactic protein-1 expression in Kupffer cells of rats with hepatic ischemia-reperfusion injury [J]. Journal of Southern Medical University, 2006, 26(08): 1180-1183.
[10]	. [J]. Journal of Southern Medical University, 2006, 26(08): 1260-1261.
[11]	ZHAO Qing, ZHAO Ke-sen Department of Pathophysiology, Southern Medical University, Guangzhou 510515, China. Effect of Bay K 8644 on arteriole smooth muscle cell membrane potential in rats with severe hemorrhagic shock [J]. Journal of Southern Medical University, 2006, 26(04): 421-424.
[12]	XU Lin, LI Zhi-liang, ZHAO Lian-you, LIU Ying-feng, LI Gong-xin, DING Ming-xue, ZHAO Yi-qiao, FU Qiang, ZHAO Xia Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China; Department of Cardiology, Tangdu Hospital, Fourth Military Medical University, Xi’an 710038, China. Effects of simvastatin on DNA synthesis in rat cardiac fibroblasts [J]. Journal of Southern Medical University, 2006, 26(02): 205-.
[13]	WANG Hui-qing, WU Sai-zhu, RUAN Yun-jun, CHENG Yu-ning, SUN Fei, RONG Zhi-yi, YIN Meng-zhuo Department of Cardiology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. A new synthetic tripeptide inhibits L-arginine transport in macrophages [J]. Journal of Southern Medical University, 2006, 26(01): 105-108.
[14]	NIU Xin-wu, FENG Jie, PENG Zhen-hui, MA Hui-qun, LIU Chao, YUAN Jing-yi. Receptor-related mechanism of proliferation inhibilion and apoptosis induetion of human tongue squamous cell line Tca8113 by retinoids [J]. Journal of Southern Medical University, 2005, 25(08): 935-941.
[15]	SHI Liang¹, LUO Ya-ling¹, LAI Wen-yan², LUO Liang³. Effects of dexamethasone on the expression of muscarinic receptor mRNA in asthmatic guinea pig airway smooth muscle and eosinophil infiltration in bronchoalveolar lavage fluid [J]. Journal of Southern Medical University, 2005, 25(08): 986-990.

Recombinant human endostatin induces apoptosis of human umbilical venous endothelial cells

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments