Abstract: Objective To study the feasibility of vector-mediated RNA interference for HER-2-positive breast cancer therapy.Methods A plasmid vector capable of mediating HER-2 RNA interference was constructed,and HER-2-positive breast cancer cell line SKBR-3 was transfected with this constructed vector.The expression of HER-2 mRNA and protein was analyzed by RT-PCR and Western blotting,and the growth and apoptosis of SKBR-3 cells was analyzed after transfection.Results The expressions of HER-2 mRNA and HER-2 protein was downregulated in response to vector-mediated HER-2 RNA interference,which also resulted in tumor cell growth inhibition and increased number apoptotic cells.Conclusion HER-2 is a good target for RNA interference and RNA interference targeting HER-2 can lead to HER-2 breast cancer cell apoptosis and growth inhibition.