Journal of Southern Medical University

Journal of Southern Medical University ›› 2006, Vol. 26 ›› Issue (04): 466-468.

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Detection of mdr1 gene by real-time fluorescence quantitative polymerase chain reaction using Taq Man-MGB probe

ZOU Ya-wei, FENG Zhi-chun, HU Bin, QIAO Ying-sa, WU Zi-liang, CHEN Fu-xiong,YE Tie-zhen Department of Pediatrics, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China; DaAn Gene Diagnostic Center, Sun Yat Sen University, Guangzhou 510089, China; Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China   

  1. 广州医学院第一附属医院儿科； 南方医科大学珠江医院儿科； 中山大学达安基因诊断中心； 广州医学院第一附属医院儿科 广东 广州 510120； 广东 广州 510282； 广东 广州 510089； 广东 广州 510120；
  • Online:2006-04-20 Published:2006-04-20

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Abstract: Primer Express 2.0 software was used to design the primers and the MGB probe. With the plasmid pHaMDR1/A containing mdr1 cDNA as the template, we established a real-time fluorescent quantitative polymerase chain reaction system, which, at the template concentration of 3.061×103 to 3.061×109 cps/ml, had a correlation coefficient of 0.988243 between template concentration and threshold cycle value. This PCR method allows sensitive, specific and quantitative detection of human mdr1 gene. 

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