Journal of Southern Medical University ›› 2006, Vol. 26 ›› Issue (04): 445-447.

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Morphological changes of ECV304 cells infected by herpes simplex virus type 2

ZHAO Hai-quan, MA Wen-li, ZHANG Ya-li, MO Xiao-yang, KE Chang-wen, ZHENG Huan-ying, ZHENG Wen-ling Institute of Molecular Biology, Southern Medical University, Guangzhou 510515, China;Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, China; South China Genomics Research Center, Guangzhou 510860, China   

  1. 南方医科大学基因工程研究所; 广东省疾病预防控制中心微生物检验所; 华南基因组研究中心 广东 广州 510515; 广东 广州 510515; 广东 广州 510300; 广东 广州 510860;
  • Online:2006-04-20 Published:2006-04-20

Abstract: Objective To observe the pathological changes and morphological alterations of ECV304 cells after the infection by herpes simplex virus type 2 (HSV-2) in vitro. Methods Passaged ECV304 cells were infected with HSV-2, TCID50 and morphological changes were observed by optical microscopy and tissue staining. Results One day after HSV-2 infeciton, swelling, rounding, and increase of thickened cytoplasmic granules occurred in the ECV304 cells, and on day-2, cell fusion was observed with weakened nuclear staining. Conclusion ECV304 cells mostly undergo necrosis after HSV-2 infection without obvious evidence of cell apoptosis. 

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