Abstract: Objective To investigate the association between the expression of human epidermal growth factor receptor(HER)-2/neu gene and prognosis of gastric cancer.Methods The expression of HER-2/neu in gastric cancer was detected by immunohistochemical technique and analyzed in relation with the follow-up and clinicopathologic data of the patients.Results Thirty-two of the 103 gastric cancer specimens(31.1 %) were positive for HER-2/neu,but whose expression was not found in benign gastric lesion and normal gastric mucosa.HER-2/neu expression was not correlated with the patients’ sex,age,tumor size and location or the degree of differentiation(P>0.05),but with the depth of invasion,clinical stage,lymph node and distant metastasis of the tumor and survival of the patients(P<0.05).Conclusion HER-2/neu expression is closely related to invasion,metastasis and prognosis of gastric cancer and can be used to evaluate the biological behavior and prognosis of gastric cancer.