Abstract: Objective To study the effects of low-power electromagnetic fields of different frequencies on proliferation and DNA damage of gallbladder cancer cells.Methods The cell growth curve was drawn and single cell gel electrophoresis performed to evaluate the proliferation and DNA damage of gallbladder cancer cells respectively after the cells were exposed to electromagnetic fields of different frequencies.Results After exposure to low-power electromagnetic fields of different frequencies(0.1-40 MHz),the cells displayed significant changes with obvious cell proliferation inhibition and DNA strand breakage.Conclusion Low-power electromagnetic fields within the range of 0.1-40 MHz may impair the DNA strand and cause inhibition of proliferation of the gallbladder cancer cells,and these effects are related to the frequency of the electromagnetic fields but not in a linear fashion.