Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV,229E and OC43 with their monoclonal antibodies

Abstract

Abstract: Objective To prepare and characterize monoclonal antibodies(mAbs) against the recombinant nucleocapsid(N) protein of 3 human coronaviruses SARS-CoV,229E and OC43 and study the antigenic relationship between the 3 N proteins.Methods BALB/c mice were immunized with the recombinant N proteins of SARS-CoV,229E and OC43 to obtain the mAbs by means of hybridoma.Screening and identification of the mAbs were performed using indirect enzyme-linked immunosorbent assay(ELISA),Western blotting and indirect immunofluorescence assay.Cross-reactivity between the N proteins of the 3 coronaviruses was analyzed with the prepared mAbs.Results The mAbs against the recombinant N proteins of SARS-CoV,229E and OC43 were obtained,which reacted specifically with the corresponding viral N protein as shown by indirect ELISA,Western blotting and indirect immunofluorescence assay.No cross-reactivity was found between the 3 N proteins.Conclusion The prepared mAbs against the recombinant N proteins may provide valuable assistance in studying antigenic relationships of N proteins between the 3 human coronaviruses.

CLC Number:

R392

QIU Li-wen,WANG Ya-di,LIAO Zhi-yong,WEN Kun,CHE Xiao-yan Central Laboratory,Zhujiang Hospital,Southern Medical University,Guangzhou 510282,China. Antigenicity analysis of nucleocapsid proteins of 3 human coronaviruses SARS-CoV,229E and OC43 with their monoclonal antibodies[J]. Journal of Southern Medical University, 2006, 26(03): 290-293.

References

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