Abstract: Objective To observe the inhibitory effect of oligodeoxynucleotides (ODN) on nuclear translocation and nuclear binding activity of nuclear factor (NF)-KB in THP-1 cells. Methods Oligodeoxynucleotides were tansfected via liposome into THP-1 cells followed by stimulation of the cells with lipopolysaccharide (LPS). Immunocytochemistry, electrophoretic mobility shift assay (EMSA) and reverse transcription (RT)-PCR were performed to detect the nuclear translocation and nuclear binding activity of NF-κB. Results Immunocytochemical results showed that after LPS stimulation of the ODN-transfected cells, NF-κB expression was still localized in cytoplasma. EMSA demonstrated inhibited nuclear binding activity of NF-κB in the ODN-transfected cells, and ODN inhibited the mRNA expression of tumor necrosis factor (TNF)-α, a NF-κB-associated inflammatory factor, as shown by RT-PCR. Conclusion ODN can inhibit the nuclear translocation and binding activity of NF-κB in THP-1 cells, whereby the transcription and expression of the related inflammation factor genes is suppressed, which shed light on a new solution for clinical treatment of acute pancreatitis.