Detection of quorum-sensing pathway and construction of LuxS gene deletion mutants of group B Streptococcus

Abstract

Abstract: Objective To detect the possible presence of AI-2 quorum-sensing pathway and construct group B Streptococcus (GBS) mutants with deletion of LuxS gene related to quorum-sensing pathway. Method V. harveyi BB170 was employed as the reporter strain to detect AI-2 pathway in GBS, and identification of LuxS homologous gene in GBS type V strain 2603 was performed by software-based analysis. LuxS gene deletion mutant ΔLusX was then constructed in GBS by means of allelic exchange and verified by Southern hybridization analysis. Results A component in the secretions of GBS could induce bioluminescence activity in the reporter strain, suggesting the presence of AI-2 quorum-sensing pathway in GBS. luxS homologous gene was detected in GBS and LuxS gene deletion mutants was successfully constructed in GBS Ia 515 and V 2603 strains. Conclusion This study establishes a bacterial model for studying the role of LuxS molecule in AI-2 quorum-sensing pathway in GBS and provides new insights into virulence regulation mechanism of GBS.

CLC Number:

R373
Q75

OU Yang-qian¹, MA Wen-li¹, LIU Cui-hua¹, SHI Rong¹, ZHENG Wen-ling². Detection of quorum-sensing pathway and construction of LuxS gene deletion mutants of group B Streptococcus[J]. Journal of Southern Medical University, 2005, 25(09): 1135-1139.

References

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