Antioxidative activity of water-soluble extracts from Fructus Polygoni Orientalis

Abstract

Abstract: Objective To study the antioxidative activity of the water-soluble extracts from Fructus Polygoni Orientalis. Methods Malondialdehyde and superoxide dismutase were measured by thiobarbituric acid assay,nitrite method,respectively.The activity of glutathione peroxidase was determined by 5,5’-dithionbis (2-nitrobenzoic acid) photometric method.Lipofuscin was determined by Sohal method in the brain of senile model mice induced by D-galactose.The extracts of 0.8,1.6 and 3.2 g·kg^-1>·d^-1 were administered to the mice separately through oral gavage daily for 7 weeks. Results Malondialdehyde content increased,activities of superoxide dismutase and glutathione peroxidase in the heart,liver and kidney decreased.Levels of lipofuscin in the brain of senile model mice induced by D-galactose increased.The three doses of the extracts could inhibit the levels of lipofuscin in the brain,malondialdehyde in the heart,liver and kidney.These results showed that there was a negative dose-effect relationship.The extracts also up-regulated the levels of superoxide dismutase and glutathione peroxidase in the heart,liver and kidney with a positive dose-effect relationship. Conclusion The water-soluble extracts exert its antioxidative activity by eliminating free hydroxyl radicals,increasing activities of superoxide dismutase and glutathione peroxidase.

CLC Number:

R932

LEI Xiao-yan¹, XU Ai-xia², GAO Xiang², GE Bin². Antioxidative activity of water-soluble extracts from Fructus Polygoni Orientalis[J]. Journal of Southern Medical University, 2005, 25(07): 820-822.

References

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