Comparison of two amine-modified chemical platforms for DNA microarray preparation

Abstract

Abstract: Objective To study two amine modification procedures for DNA microarray preparation based on polymeric coatings. Methods One of the proposed approaches utilized poly-amine coating of silanized slides activated by 1,4-phenylene diisothiocyanate,and the other employed acrylic acid-co-acrylamide copolymer and 1-(3-dimethylamino propyl)-3-ethylcar- bodiimide hydrochloride/N-hydroxysuccinimide as the coating agents and activator,respectively.The modified slides were used for preparing λ phage DNA microarrays,whose properties were analyzed by hybridization. Results Formation of dendri- meric structure and polymer was observed on the surface of the slides.The signal spots in uniform,steady and regular shape,in comparison with the commercial CMT-GAPS slides,indicated successful manufacture of the microarrays. Conclusion The two platforms are suitable for microarray preparation,and the method of acrylic acid-co-acrylamide copolymer modification is more preferred.

CLC Number:

Q789

WU Qing-hua¹, MA Wen-li¹, WANG Hong-min¹, MAO Xiang-ming¹, ZHANG Bao¹, LI Ling¹, ZHENG Wen-ling². Comparison of two amine-modified chemical platforms for DNA microarray preparation[J]. Journal of Southern Medical University, 2005, 25(07): 794-798.

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