Expression of aquaporin-4 in isolated and purified rat alveolar typeⅡcells

Abstract

Abstract: Objective To explore the expression of aquaporin-4 (AQP4) in isolated and purified rat alveolar typeⅡcells. Methods Rat alveolar typeⅡcells were isolated,purified and identified by special staining and electron microscopy.The distribution of AQP4 in alveolar typeⅡcells was observed by immunocytochemistry and immunoelectron microscope with affinity-purified antibodies to human AQP4. Results Immunocytochemistry showed staining for AQP4 in rat alveolar type Ⅱ cells,and immunoelectron microscope demonstrated the presence of AQP4 on the membrane of the cells. Conclusion AQP4 is expressed in alveolar typeⅡcells in rats,and may play a role in water transportation in the respiratory system.

CLC Number:

R-33

LIU Lin, LI Tao-ping. Expression of aquaporin-4 in isolated and purified rat alveolar typeⅡcells[J]. Journal of Southern Medical University, 2005, 25(07): 784-786.

References

[1] Borgnia M, Nielsen S, Engel A, et al. Cellular and molecular biology of the aquaporin water channel [J]. Annu Rev Biochem, 1999, 68:425-58.
[2] Dobbs LG, Gonzalez R, Williams MC. An improved method for isolating type Ⅱ cells in high yield and purity [J]. Am Rev Respir Dis, 1986, 134(1): 141-45.
[3] Mason RJ, Walker SR, Shields BA, et al. Identification of rat alveolar type Ⅱ epithelial cells with a tannic acid and polychrome stain [J].Am Rev Respir Dis, 1985, 131 (5): 786-88.
[4] 刘琳,李涛平.急性呼吸窘迫综合征大鼠肺泡Ⅱ型上皮细胞的超微结构观察[J].第一军医大学学报,2003,23(7):690-95.Liu L, Li TP. Ultrastructure of type Ⅱ alveolar epithelial cells in rats with acute respiratory distress syndrome [J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2003, 23(7): 690-5.
[5] Verkman AS, Matthay MA, Song Y. Aquaporin water channels and lung physiology[J]. Am J Physiol, 2000, 278(5): L867-L79.
[6] Lee MD, King LS, Agre P. The aquaporin family of water channel proteins in clinical medicine[J]. Medicine (Baltimore), 1997, 76(3):141-56.
[7] Nielsen SL, King S, Christensen BM, et al. Aquaporins in complex tissues: Ⅱ. Subcellular distribution in respiratory and glandular tissues of rat[J]. Am J Physiol, 1997, 273(5 Pt 1): C1549-C61.
[8] Bai C, Fukuda N, Song Y, et al. Lung fluid transport in aquaporin- 1and aquaporin-4 knockout mice [J]. J Clin Invest, 1999, 103(4):555-61.
[9] Effros RM, Darin C, Jacobs ER, et al. Water transport and the distribution of aquaporin-1 in pulmonary air spaces [J]. J Appl Physiol, 1997, 83(3): 1002-16.
[10] Kreda SM, Gynn MC, Fenstermacher DA, et al. Expression and localization of epithelial aquaporins in the adult human lung [J].Am J Respir Cell Mol Biol, 2001, 24(3): 224-34.
[11] King LS, Nielsen S, Agre P. Aquaporins and the respiratory system:advice for a lung investigator[J]. J Clin Invest,2000, 105(1): 15-6.
[12] King LS, Yasui M. Aquaporins and disease: lessons from mice to humans[J]. Trends Endocrinol Metab, 2002, 13(8): 355-60.

[1]	YUAN Qiang, LIU Da-lie, YUAN Ji-long, SHAN Lei Department of Plastic and Reconstructive Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Establishment of rabbit orbital hypertelorism model and evaluation of effects of surgical correction [J]. Journal of Southern Medical University, 2006, 26(10): 1476-.
[2]	CUI Yan-zhi1, HAN Ya-guang1, LUO Rong-cheng1, YANG Hai-qing2, JIANG Da3 1Department of Oncology, Nanfang Hospital, 3Department of Statistics, Southern Medical University, Guangzhou 510515, China; 2Department of Oncology, Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, China. Establishment of an rat model of tumor-associated depression [J]. Journal of Southern Medical University, 2006, 26(10): 1513-.
[3]	. [J]. Journal of Southern Medical University, 2006, 26(08): 1209-1210.
[4]	LI Hao, PENG Han-wei, ZENG Zong-yuan, GUO Zhu-ming State Key Laboratory of Oncology in Southern China, Department of Head and Neck, Oncology Center, Sun Yat-sen University, Guangzhou 510060, China. Research of modified rat laryngeal transplantation model [J]. Journal of Southern Medical University, 2006, 26(07): 994-.
[5]	. [J]. Journal of Southern Medical University, 2006, 26(07): 1069-1070.
[6]	CHEN Xiao-lin,HE Xiao-hong,ZHANG Yan,QIAN Yue-tao,LIANG Lu-guang,FANG Dian-qiu,ZHAN Cheng-yang,ZHENG Zhen-sheng,MA Hong Department of Cardiology,First Affiliated Hospital,Sun Yat-sen University,Guangzhou 510089,China. Establishment of a pig model of chronic ally enhanced external counterpulsation [J]. Journal of Southern Medical University, 2006, 26(05): 613-614.
[7]	DONG Gao-hong1,ZHANG Zhao-da2,HU Wei-ming2,TANG Yong2,LI Jian-shui2 1Center of Organ Transplantation,First Affiliated Hospital of Jinan University,Guangzhou 510630,China;2West China Hospital of Sichuan University,Chengdu 610041,China. Establishment of whole pancreaticoduodenal allotransplantation model with portal venous drainage and enteric drainage in pigs [J]. Journal of Southern Medical University, 2006, 26(05): 626-628.
[8]	LI qi1,HUANG De-liang2 1Institute of Otolaryngology,2Department of Otolaryngology-Head and Neck Surgery,General Hospital of PLA,Beijing 100853,China. Expression of aquaporin-1 in a guinea pig model of labyrinth destruction [J]. Journal of Southern Medical University, 2006, 26(05): 664-666.
[9]	. [J]. Journal of Southern Medical University, 2006, 26(05): 707-708.
[10]	LIN Xiao-jing, ZOU Fei, LI Ya-jie, WANG Bin, HE Wen-jing, ZHAO Zhi-rong, ZHU Shun-fang Nanfang Hospital, Institute of Public Hygiene and Tropical Medicine, Southern Medical University, Guangzhou 510515, China. Establishment of a rat model of severe heatstroke complicated with endotoxemia [J]. Journal of Southern Medical University, 2006, 26(01): 86-89.
[11]	XIONG Bing¹, CHEN Hua-de¹, LAI Wen¹, XIONG Shi-qiu², ZHEN Shao-yi¹, GAO Hui¹, BIAN Hui-ning¹, LIU Zu-an¹. Establishing a parabiosis animal model of two-way paradigm [J]. Journal of Southern Medical University, 2005, 25(12): 1511-1513,1516.
[12]	FU Rui-bin¹, WU Ping-sheng², SONG Yun-feng¹, QIU Jian¹, DAI Tie-ying², LI Jian-hua², XIU Jian-cheng². Construction of eukaryotic expression vectors of two mutants of hypoxia-inducible factor-1 and their expressions in human microvascular endothelial cells [J]. Journal of Southern Medical University, 2005, 25(11): 1348-1351.
[13]	HU Jing¹, YU Shou-yi¹, KAN Biao², LIU Zhi-hua³. Construction and characterization of mutant Enteroaggregative E. coli O42 strain with high-pathogenicity island deletion [J]. Journal of Southern Medical University, 2005, 25(11): 1352-1356.
[14]	DENG Hong, ZOU Fei, LUO Hai-ji. Differentiation of neonatal rat striatal neural stem cells induced by all-trans retinoic acid [J]. Journal of Southern Medical University, 2005, 25(11): 1357-1360,1374.
[15]	QIU Xiao-zhong¹, YU Lei¹, LIAO Hua¹, ZHANG Li-sheng², QIN Jian-qiang¹, YANG Jun¹, OUYANG Jun¹. Oxidative stress-induced differentiation of L6 myoblasts [J]. Journal of Southern Medical University, 2005, 25(11): 1384-1386.