c-myc gene silencing in K562 cells with RNA interference

Abstract

Abstract: Objective To study the inhibitory effect of small interfering RNA (siRNA) targeting c-myc gene in K562 cells. Methods siRNAs targeting the site 1357 of c-myc mRNA was designed and synthesized. In vitro cultured K562 cells were transfected with lipofectamine^TM 2000 and the inhibitory effect was detected by reverse transcriptase (RT)-PCR, cell count, MTT assay and fluorescence-activated cell sorting. Results Compared with the negative and blank control group, the transfection group showed marked decrease in the c-myc expression and the K562 cells exhibited increased apoptosis rate. Conclusion RNA interference can effectively inhibit c-myc expression and induce apoptosis in K562 cells.

CLC Number:

YUE Feng¹, MA Wen-li¹, SONG Yan-bin¹, SHI Rong¹, ZHANG Bao¹, ZHENG Wen-ling². c-myc gene silencing in K562 cells with RNA interference[J]. Journal of Southern Medical University, 2005, 25(06): 647-650.

References

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