Journal of Southern Medical University ›› 2005, Vol. 25 ›› Issue (04): 476-478.

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Purification of a small peptide SV-PP-3 from the venom of Agkistrodon blomhoffii siniticus and its inhibition of platelet aggregation

XU Wei, WU Shao-yu, ZHANG Jia-jie, LI Zhi-qin, WU Shu-guang   

  1. 南方医科大学药物研究所, 广东, 广州, 510515
  • Online:2005-04-20 Published:2005-04-20

Abstract: Objective To investigate the inhibitory effect of the small peptide SV-PP-3 from the venom of Agkistrodon blomhoffii siniticus on platelet aggregation. Methods The small peptide SV-PP-3 was isolated and purified by centrifugation at low temperature, sephadex G-75 column chromatography and reverse-phase (HPLC) on C-18 column. The platelet aggregation induced by adenosine diphosphate (ADP) was measured by LBY-NJ aggregometer. Results SV-PP-3 was purified from the venom of Agkistrodon blomhoffii siniticus, which inhibited the platelet ADP-induced platelet aggregation in a dose-dependent manner. Conclusion SV-PP-3 can inhibit ADP-Induced platelet aggregation.

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