Abstract: Objective To identify the active components of compound dandelion enema, a preparation from 7 traditional Chinese herbal drugs for treatment of gynecological diseases. Methods Three-dimensional high-performance liquid chroma- tography (3D-HPLC) was employed to separate the ethyl acetate extract of compound dandelion Enema, and HPLC combined with mass spectrum (MS) analysis used for chromatographic fingerprinting. Result By comparing the ionic fragments of MS and retention time of each peak, the main active components in compound dandelion enema were determined, including caffeic acid, ferulic acid and protocatechualdehyde. Conclusion HPLC coupled with mass spectroscopy can be used for qualitative analysis of compound dandelion enema.