Abstract: Objective To examine the expressions of P16 and nm23-H₁ protein in nasopharyngeal carcinoma (NPC) and explore their association with clinicopathology and the level of argyrophilic protein of the nucleolar organizer regions (AgNORs) of peripheral blood T lymphocyte (T-AgNORs) before radiotherapy.Methods SP immunohistochemistry was used to detect P16 and nm23-H₁ expressions in 65 cases of NPC, and the level of T-AgNORs was measured before radiotherapy.Results The positivity rate of P16 and nm23-H₁ protein in NPC was 24.6% (16/65) and 61.5% (40/65), respectively. The positivity rates of P16 and nm23-H₁ protein in patients in T₁ and T₂ stages were higher than those in patients in T₃ and T₄ stages, but the difference was not statistically significant (P>0.05). No significant association of P16 expression was noted with lymph node metastasis and post-radiotherapy distant metastasis (P>0.05). In patients with regional lymph node and distant metastases, the positivity rates for nm23-H₁ were 51.2% (21/41) and 33.3% (5/15), which were significantly lower than those in patients without local lymph node (79.2%, 9/24)(X²= 4.99, P<0.05) or distant metastasis (70%, 35/50)(X²=7.56, P<0.01). The levels of T-AgNORs in P16-and nm23-positive cases were higher than those in the negative cases (t=5.721, P<0.001).Conclusions nm23-H₁ expression in NPC tissue is correlated with NPC metastasis and may be useful for clinical prediction of local lymph node and distant metastases after radiotherpay. nm23 and p16 expressions are also correlated with the level of T-AgNORs, which probably indicate the immune functions of the patients.