Abstract: Objective To investigate the effects of Emu(Dromaius novaehollandiae) eggshell produced in Guangdong on sexual ability of male mice. Methods Mating test was performed in mature male mice after intragastric administration of Emu eggshell for 21 days. The relative weight of the sexual organs in immature male mice was measured after administration for 30 days. A model of sexual dysfunction of male mice was established by repeated hanging stress, and the mating ability of the male mice was examined. Results Emu eggshell administration significantly shortened the latency time and increased mating frequency in mature male mice, and the relative weight of the sexual organs of immature male mice was increased and sexual function recovery was promoted. Conclusion Emu eggshell produced in Guangdong can enhance sexual function and promote the growth and development of the sexual organs of male mice to a certain extent.