Cantharidin regulates intracellular ATP in G1/S-phase renal tubule epithelial cells

Abstract

Abstract: Objectives To study the mechanism of cantharidin in protecting F-actin microfilaments from disruption by hypoxic damage by observing the effects of cantharidin on intracellular ATP metabolism in G₁/S-phase renal tubule epithelial cells (RTECs).Methods G₁-phase RTECs were divided into cantharidin-treated group,exposed to sodium cyanide (CN) and cantharidin,hypoxic-group with CN exposure and non-treated control group. ATP levels were measured in the 3 groups with high-performance liquid chromatography. Results The concentration of CN exposure for 1 h,ATP level in the RTECs with cantharidin treatment were significantly higher than that in both hypoxic and non-treated control groups (14.50±0.26 mmol/g protein,4.25±0.11 mmol/g protein,8.58±0.13 mmol/g protein,respectively,P<0.01).Conclusion Cantharidin prevents the disruption of the actin cytoskeleton in hypoxic damage by preventing abnormal intracellular ATP metabolism.

CLC Number:

SHEN Qing¹, YAO Yu-jia², LI Ru-ying¹, DING Cui-ling¹, HOU Li-chao³, SUN Xu-de³. Cantharidin regulates intracellular ATP in G₁/S-phase renal tubule epithelial cells[J]. Journal of Southern Medical University, 2004, 24(10): 1192-1193,1196.

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Cantharidin regulates intracellular ATP in G₁/S-phase renal tubule epithelial cells

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