Abstract: Objective To evaluate the value of ^99mTc-MIBI brain single-photon emission computerized tomography (SPECT) in diagnosis of glioma.Methods Fifty-nine patients with glioma,6 with brain abscess and 9 healthy controls underwent ^99mTc-MIBI brain SPECT,and the diagnostic indices such as the sensitivity,specificity and accuracy were calculated. The tumor to non-tumor (T/NT) ratios were calculated according to the region of interest (ROI) and compared between the glioma group,healthy control group and brain abscess group by t test. Results Among the 59 cases of glioma,51 showed positive results in ^99mTc-MIBI SPECT,along with one of the healthy controls and 4 of brain abscess patients. The sensitivity,specificity and accuracy of the diagnosis were 86.4%,66.7% and 82.4%,respectively. The T/NT ratio of brain glioma group was 2.6±1.2,significantly higher than that of normal group (t=3.6199,P<0.001) and brain abscess group (t=2.1327,P<0.05).Conclusion ^99mTc-MIBI brain SPECT is sensitive for diagnosis of brain glioma,and can distinguish malignant from benign lesions effectively.