Abstract: Objective To establish a fast and simple method for screening mutant yeast strains. Materials and Methods Homologous recombination technique was used to detect mutant yeast strains in yeast genomic library,with the green fluore- scent protein gene as the reporter gene in the transposon. Results The strains that emitted green fluorescence were isolated,indicating that the gfp gene was inserted into the yeast genome by homologous recombination.Conclusion This study established a useful method for functional genome study by homologous recombination technique,and provide an alternative for gene therapeutic drug development.