Effect of astrocyte-conditioned medium on tert-butyl hydroperoxide-induced apoptosis of PC12 cells

Abstract

Abstract: Objective To investigate the effect of astrocyte-conditioned medium (ACM) on PC12 cell apoptosis induced by reactive oxygen species tert-butyl hydroperoxide (tbOOH). Methods PC12 cells attacked by tbOOH were cultured with Sprague Dawley rat cerebral cortex ACM, and the cell apoptosis was observed by fluorescent microscopy and transmission electron microscopy. The cell apoptosis rate was evaluated by flow cytometry and malondialdehyde (MDA) concentration measured using thiobarbituric acid (TBA)-reacting substances (TRABS). Result TbOOH induced PC12 cell apoptosis, and ACM significantly decreased the apoptosis rate and MDA concentration in tbOOH-treated cells. Conclusion ACM may increase the anti-oxidation capacity of PC12 cells and inhibit cell apoptosis induced by tbOOH.

CLC Number:

R392.2

MO Yong-yan¹, XING Fei-yue², ZHANG Bao³, CHEN Yuan³, JIANG Yong¹. Effect of astrocyte-conditioned medium on tert-butyl hydroperoxide-induced apoptosis of PC12 cells[J]. Journal of Southern Medical University, 2004, 24(09): 998-1000.

References

[1] Sayre LM, Smith MA, Perry G. Chemistry and biochemistry of oxidative stress in neurodegenerative disease[J]. Curr Med Chem, 200 l,8(7): 721-38.
[2] 莫永炎,陈瑗,周玫,等.星形神经胶质细胞对PC12神经元生长发育的影响[J].神经解剖学杂志,2000,16(3):247-50.Mo YY, Chen Y, Zhou M, et al. Effects of cultured astrocytes from rat cerebral cortex on the development of PC12 cells[J]. Chin J Neuroanat, 2000, 16(3): 247-50.
[3] 莫永炎,陈瑷,周玫.星形神经胶质细胞条件培养液对活性氧致的神经元毒的保护作用[J].细胞生物学杂志,2000,22(1):39-42.Mo YY, Chen Y, Zhou M. The protective effects of conditioned medium from astrocytes on neurons against reactive oxygen induced toxicity[J]. Chin J Cell Biol, 2000, 22(1): 39-42.
[4] 梁卫江,黄卓垣,丁彦青,等.蛋白合成抑制剂促进TNFα诱导大肠癌细胞凋亡[J].第一军医大学学报,1999,19(2):115-6.Liang WJ, Huang ZY, Ding YQ, et al. Promotion of TNFa-induced apoptosis with cycloheximide in colorectal carcinoma cell line LoVo[J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 1999, 19(2): 115-6.
[5] 王克万,漆松涛,杨志焕,等.培养大鼠星形胶质细胞牵张损伤后超微结构的变化[J].第一军医大学学报,2002,22(8):687-9.Wang KW, Qi ST, Yang ZH, et al. Ultrastructural features of cultured rat cortical astrocytes with stretch-induced injury[J]. J First Mi’ Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2002, 22(8): 687-9.
[6] Kuo PC, Abe KY. Interleukin 1-induced production of nitric oxide inhibits benzenetriol-mediated oxidative injury in rat hepatocytes[J].Gastroenterology, 1995, 109(2): 206-16.
[7] Behl C, Davis JB, Lesley R, et al. Hydrogen peroxide mediates amyloid beta protein toxicity[J]. Cell, 1994, 77(6): 817-27.
[8] 迟先煊,郭畹华,庆宏,等.过氧化氢诱发小脑皮质神经元凋亡[J].神经解剖学杂志,1998,14(1):19-22.Chi XX, Guo WH, Qing H, et al. Hydrogen peroxide induces apoptosis of cerebellar cortical neuron[J]. Chin J Neuroanat, 1998, 14(1):19-22.
[9] Bergi E. The significance of lipofuscin in the aging process, especially in the neurons[J]. Aging, 1982, 18(1): 15-21.