Journal of Southern Medical University ›› 2004, Vol. 24 ›› Issue (09): 995-997.

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Renaturation of thermolabile hemolysin inclusion bodies expressed by Vibrio parahaemolyticus

LI Zhi-feng, NIE Jun, DAI Ying-chun, CHEN Qing, YU Shou-yi   

  1. 第一军医大学流行病学教研室, 广东, 广州, 510515
  • Online:2004-09-20 Published:2004-09-20

Abstract: Objective To clone and construct expression vector pET32a+-tlh to acquire biologically functional thermolabile hemolysin of Vibrio parahaemolyticus. Methods tlh gene was cloned and the expression vector pET32a+-tlh constructed. The tlh gene of Vibrio parahaemolyticus was expressed in DE3 in the form of inclusion body, which was resolved in 8 mol/L urea followed by purification of the fusion protein using affinity chromatography and renaturation through gradient dialysis, protein concentration reduction and oxidoreduction. Results and Conclusion The purified and renatured protein possessed hemolytic and immunogenic activities.

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