Phospholipase C-γ1 dynamic mRNA expression patterns in rats during early postnatal period

Abstract

Abstract: Objective To investigate the expression of phospholipase C-γ1 (PLCG1) mRNA in rats during early postnatal period. Methods Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the expression of PLCG1 mRNA in 28 samples extracted from the liver, lung, kidney and brain of rats aged 1, 3, 5, 7 days, and 2, 3, 5 weeks. Specific PLCG1 product and GAPDH product as the internal control were both amplified by RT-PCR, and the ratio of their integral optical density was calculated to estimate the relative mRNA expression of PLCG1. Results PLCG1 was expressed in rat liver, lung, kidney and brain at the 7 postnatal time points, and the expression varied significantly with time and between the different organs (P<0.01), virtually undetectable in the liver on postnatal day 1 and reaching the highest level in the brain tissues on postnatal day 7. Conclusion The differences in PLCG1 expression in various organs and development periods suggest that PLCG1 is involved in cell proliferation and differentiation during the early development of rats.

CLC Number:

LIU Zhong-ying¹, LUO Shen-qiu¹, DENG Fan¹, ZHAO Yong-zhong¹, LI Xi-ping². Phospholipase C-γ1 dynamic mRNA expression patterns in rats during early postnatal period[J]. Journal of Southern Medical University, 2004, 24(09): 987-990.

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