Effects of recombinant Helicobacter pylori catalase on oxidative stress in colonic mucosal epithelial cells

Abstract

Abstract: Objective To investigate the effects of recombinant Helicobacter pylori catalase (rHpCAT)on oxidative stress in rat colonic mucosal epithelial cells. Methods Oxidative stress model was established by hydroxyl generated from Fenton reaction in cultured colonic mucosal epithelial cells isolated from normal rats, in the model of which the effects of rHpCAT were observed. The cells were divided into normal control, model, 5-aminosalicvlic acid (5-ASA, 0.1 mmol/L), and rHpCAT (1×10⁵, 1×10⁶, and 1×10⁷ U/kg, respectively) groups. At the end of the experiment, the content of lactic dehydrogenase (LDH), malondialdehyde (MDA), myeloperoxidase (MPO), glutathione peroxidase (GSH-Px), catalase (CAT) and, superoxide dismutase (SOD) were detected in the culture supernatant. Results The contents of LDH, MDA and MPO were elevated while those of GSH-Px, CAT and SOD reduced in the model group. rHpCAT at different doses reduced the release of LDH, depressed the contents of MDA and MPO, and increased the contents of GSH-Px, SOD and CAT. Conclusion rHpCAT has protective effects against rat colonic mucosal oxidative damage.

CLC Number:

WU Jin-bao¹, WANG Ji-de¹, WANG Qun-yin¹, LÜ Yong-hui², XU Jun¹, YE Fang-peng¹, NAN Qing-zhen¹, LI Liang-ren¹, REN Yue-xin¹, ZHANG Ya-li¹. Effects of recombinant Helicobacter pylori catalase on oxidative stress in colonic mucosal epithelial cells[J]. Journal of Southern Medical University, 2004, 24(09): 1045-1047.

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