Hominis placenta promotes melanocyte proliferation,melanin synthesis and tyrosinase activity in vitro

Abstract

Abstract: Objective To investigate the effects of Hominis placenta on melanocytes and tyrosinase activity in vitro. Methods MTT assay was used to assess the proliferation of melanocytes treated with Hominis placenta. Hunt method was employed to determine melanin synthesis and the oxidation rate of DL-dopa to evaluate the tyrosinase activity. Results Hominis placenta promoted melanocyte proliferation (P<0.01) and significantly increased the cell number (P<0.01), melanin synthesis (P<0.01), with the optimal concentration of 0.1g/L (P<0.01), and also enahnced tryosinase activity (P<0.01) at the optimal concentration of 1 g/L (P<0.01). Conclusion Hominis placenta promotes proliferation, melanin synthesis and tyrosinase activity of the melanocytes.

CLC Number:

R285.5

YANG Liu, DENG Yan. Hominis placenta promotes melanocyte proliferation,melanin synthesis and tyrosinase activity in vitro[J]. Journal of Southern Medical University, 2004, 24(09): 1040-1041,1044.

References

[1] Eisinger M, Marko O. Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and cholera toxin[J].Proc Natl Acad. Sci USA, 1982, 79:2018
[2] 赵辨,黄秋玲,毕志刚,等.人正常黑素细胞体外纯培养及其细胞生物学鉴定[J].临床及实验研究(Chin Lab Res),1991,(5):226-8.
[3] 龚志锦,詹荣渊,主编.病理组织制作和染色技术[M].上海科学技术出版社,1994.183-5.
[4] Mosman T. Rapid colorimetric assay for celluar growth and survival:application to proliferation and cytotoxicity assays[J]. J Immunol Methods, 1983, 65: 55.
[5] 邓燕,杨柳.当归对体外黑素细胞和酪氨酸酶的激活作用[J].第一军医大学学报,2003,23(3):239-41.Deng Y, Yang L. Effect ofA ngelica sinensis (Oliv.) on melanocytic proliferation, melatin synthesis and tyrosinase activity in vitro[J]. J Fisrt Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2003, 23(3): 239-41.
[6] Hunt G, Todd C, Cresswell JE. α-melanocyte stimulating hormone and its analogue Nie4Ophe7α-MSh affect morphology, tyrosinase activity and melanogenesis in cultured human melanocytes[J]. J Cell Sci,1997, 107:205-211
[7] Matsuda H, Higashino M, Nakai Y. Studies of cuticle drugs from natural sources. Ⅳ. Inhibitory effects of Arctostaphylos plants on melanin biosynthesis[J]. Bio Pharm Bull, 1996, 19(1): 153-6.
[8] 邓燕,杨柳,安胜利.不同浓度刺蒺藜对黑素细胞和酪氨酸酶的作用[J].第一军医大学学报,2002,22(11):1017-9.Deng Y, Yang L, An SL. Effect ofTribulus terrestris L decoction of different concentrations on tyrosinase activity and the proliferation of melanocytes[J]. J Fisrt Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2002, 22 (11): 1017-9.
[9] Kubo I. Yolokawa Y, Kinst-Hori I. Tyrosinase inhibitors from Bolivian medicinal plants[J]. J Nat Prod, 1995, 58(5): 739-43.
[10] Smith B, Selby P, Southgate J, et al. Detection of melanoma cells in peripheral blood by mesns of reverse transcriptase and polymerase chain reaction[J]. Lancet, 1991, 338(8777): 1227-9.

[1]	MO Zhi-xian1,AN Sheng-li2,ZHOU Ji-yin1 1College of Traditional Chinese Medicine,2Department of Biological Statistics,Southern Medical University,Guangzhou 510515,China. Effects of Caulis Sinomenii and sinomenine on morphine-induced place preference and brain histamine level in mice [J]. Journal of Southern Medical University, 2006, 26(12): 1709-1713.
[2]	. [J]. Journal of Southern Medical University, 2006, 26(09): 1376-1377.
[3]	XIE Wei1, LI Chang-zheng2, BAO Yong2, YU Li-jian2 Department of Traditional Chinese Medicine, Nanfang Hospital1, School of Traditional Chinese Medicine2, Southern Medical University, Guangzhou 510515, China. Saikosaponins inhibit increased glutamate and GABA expressions in the hippocampus of pentetrazole-induced slow kindling rats [J]. Journal of Southern Medical University, 2006, 26(08): 1132-1135.
[4]	YIN Rui-xue, LU Bing-xun, WANG Li-xin, FAN Jian-zhong, LU Chang-jun, LIU Yi-xing Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. Nestin activation after rat cerebral ischemia-reperfusion injury and its changes in response to Tongxinluo treatment [J]. Journal of Southern Medical University, 2006, 26(06): 777-779.
[5]	ZHANG Qun, LEI Lin-sheng, ZHU Zheng-guang, YU Chuan-lin, WU Shu-guang Institute of Pharmaceutical Sciences, School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, China. Ganoderma polysaccharides antagonize prostaglandin E2-induced suppression of murine splenocyte IFN-γ and TNF-α mRNA expression [J]. Journal of Southern Medical University, 2006, 26(06): 780-783.
[6]	. [J]. Journal of Southern Medical University, 2006, 26(05): 701-702.
[7]	XIE Wei, BAO Yong, YU Li-jian, CHEN Yu-yao School of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China. Effect of saikosaponins on glial fibrillary acidic protein expression in hippocampal astrocytes of pentetrazole-induced chronic kindling rats [J]. Journal of Southern Medical University, 2006, 26(04): 452-455.
[8]	CHEN Da-li, HUANG Tao School of Traditional Chinese Medicine, Southern Medical University, Guangzhou510515, China. In vivo and in vitro anti-tumor effect of Sanjie Kangliu Decoction [J]. Journal of Southern Medical University, 2006, 26(04): 479-482.
[9]	CHEN Guo-bao, WEI Lian-bo, XIAO Wei, LONG Hai-bo Center of Integrated Traditional and Western Nephrology, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China. Effect of Shenkangwan on mesangial cell NO and TGF-β1 excretion in rats with early diabetic nephropathy [J]. Journal of Southern Medical University, 2006, 26(04): 526-528.
[10]	XIE Wei, BAO Yong, YU Li-jian, HOU Guang-nan, TAN Hong-xiang Department of Internal Medicine, School of Traditional Chinese Medicine, Center of EEG, Department of Neurology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China; Department of Neurological Rehabilitation, Guangzhou Children’s Hospital, Guangzhou 510120, China. Effect of saikosaponins on epileptic seizure and EEG in pentetrazole-induced chronic kindling rats [J]. Journal of Southern Medical University, 2006, 26(02): 177-180.
[11]	TAN Xian-hui, QU Hong-da, PENG Kang, CHEN Yu-yao, TONG Li, SHEN Jian-gang, ZHU Chuan-wu College of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510515, China; School of Traditional Chinese Medicine, Chinese University of Hong Kong, Hong Kong, China. Effects of Buyanghuanwu decoction on nerve proliferation in rats with sequelae of ischemic stroke [J]. Journal of Southern Medical University, 2006, 26(02): 189-192.
[12]	ZHANG Tao, RONG Xin-zhou, YANG Rong-hua, LI Tian-zeng, XU Ying-bin Department of Burns, Guangzhou First Municipal People’s Hospital, Guangzhou 510180, China; Department of Burns, First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, China. Effect of asiaticoside on the expression of transforming growth factor-beta mRNA and matrix metalloproteinases in hypertrophic scars [J]. Journal of Southern Medical University, 2006, 26(01): 67-70.
[13]	MO Zhi-xian¹, LEUNG Wing-nang², YUNG Kin-lam³. Changes in cAMP and cGMP levels in neonatal rat histaminergic neurons of tuberomammillary nucleus following 48-hour morphine exposure and effects of sinomenine intervention [J]. Journal of Southern Medical University, 2005, 25(09): 1105-1108.
[14]	YU Lin-Zhong, WU Jie-yong, LUO Jia-Bo, CHEN Yu-rao, LIN Hui. Effects of different compositions of Gegenqinlian decoction on experimental shigellosis in rabbits [J]. Journal of Southern Medical University, 2005, 25(09): 1132-1134.
[15]	JIANG Wen-ling, LUO Xian-ling, KUANG Su-juan. Effects of Alternanthera philoxeroides Griseb against dengue virus in vitro [J]. Journal of Southern Medical University, 2005, 25(04): 454-456.