Impact of early-stage hepatic ischemia-reperfusion injury on other organs of rats

Abstract

Abstract: Objective To investigate the influence of hepatic ischemia-reperfusion on other organs of rats during the early stage of injury. Methods Twenty-five healthy male SD rats were randomized equally into 5 groups according to the different time points in the course of hepatic vascular occlusion and reperfusion, namely pre-occlusion, the end of occlusion, and after 0.5, 2.0 and 6.0 h reperfusion, respectively. In each group the rats were killed to obtain samples at the specified time points. The contents of malondialdehyde (MDA) and superoxide dismutase (SOD) in the lung, kidney, pancreas and heart were measured in pre-occlusion and 6-hour reperfusion groups. The triphosphate (ATPase) activity in heart tissues, the contents of MDA and the activity of SOD in blood and the blood biochemistry were observed in different groups. Results The activity of SOD and ATPase were decreased (P<0.05) after the ischemia-reperfusion as compared with those before ischemia, but the contents of MDA, ALT, AST BUN, AMY and CK-MB increased significantly (P<0.05). Conclusion Liver ischemia-reperfusion can induce injury to other organs at the early stage of reperfusion.

CLC Number:

R657.31

YANG Jin-cheng, JI Xi-qing, LI Chao-long, LIN Jian-hua, LIU Xing-guo. Impact of early-stage hepatic ischemia-reperfusion injury on other organs of rats[J]. Journal of Southern Medical University, 2004, 24(09): 1019-1022.

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