Identification of two new strains of norovirus in Guangzhou

Abstract

Abstract: Objective To identify the molecular biological characteristics of human caliciviruses (HuCVs) and new strains of norovirus (NV) detected in Guangdong Province. Methods HuCVs were detected from the stool specimens by reverse transcription-PCR (RT-PCR), and the PCR products were cloned, transformed, and blasted with Genbank.The nucleotide sequence homology was also evaluated with PHYLIP package, and the phylogenetic tree was generated by Treeview. Results HuCVs were detected from 18 out of 206 stool specimens from children with viral diarrhea (8.74%), which were all confirmed subsequently as NVs. Two strains HuCV/NVGⅡ 003/2003/CHN(32282) and HuCV/NVGⅡ 004/2003/CHN(32283) from the selected 6 NVs strain were identified as new NV strains. Conclusion New NV strains in consistency with the blood-type distribution of Chinese are present in Guangzhou, which have not been reported in other countries.

CLC Number:

R373

DAI Ying-chun¹, NIE Jun¹, LIU Yi¹, LI Zhi-feng¹, BAI Yang², LI Jian-dong¹, YU Shou-yi¹, JIANG Xi². Identification of two new strains of norovirus in Guangzhou[J]. Journal of Southern Medical University, 2004, 24(09): 1016-1018,1022.

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