Difference in gene expression profile of human polymeric immunoglobulin receptor-transfected mouse nasopharyngeal epithelial cells before and after EBV infection

Abstract

Abstract: Objective To study the gene expression profile of human polymeric immunoglobulin receptor gene (hpIgR)-transfected mouse nasopharyngeal epithelial cells transformed with n, n’-dinitrosoperazine (TMNE) before and after EBV infection using cDNA array and investigate the role of Epstein-Barr virus (EBV) infection in the tumorigenesis of nasopharyngeal carcinoma (NPC). Methods The total RNAs of hpIgR-transfected TMNE cells before and after EBV infection were extracted, reversely transcribed, and labeled with α-³²P-dATP. The cDNA probes were hybridized to the AtlasTM mouse cancer array 1.2, and the signals analyzed by AtlasImage^TM software. Results Twenty-five genes differentially expressed in cells before and after EBV infection, including 23 up-regulated genes and 2 down-regulated genes. Conclusions The gene expression profile of hpIgR-transfected TMNE cells may change after EBV infection, suggesting that these genes are probably involved in the tumorigenesis and progression of NPC.

CLC Number:

R730.231

WANG Shuang, LÜ Li-chun, LI Hong, JIANG Pei-zhou, YAO Kai-tai. Difference in gene expression profile of human polymeric immunoglobulin receptor-transfected mouse nasopharyngeal epithelial cells before and after EBV infection[J]. Journal of Southern Medical University, 2004, 24(09): 1001-1005.

References

[1] 游绍进,姚开泰,曹亚,等.Epstein-Barr病毒潜伏状态与鼻咽癌的关系[J].中华肿瘤杂志,1996,18(1):23-6.You SJ, Yao KT, Cao Y, et al. Latency of Epstein-Barr virus and its relationship to nasopharyngeal carcinomas[J]. Chin J Oncol, 1996,18(1): 23-6.
[2] Carulli JP, Artinger M, Swain PM, et al. High throughput analysis of differential gene expression[J]. J Cell Biochem Suppl, 1998,30 (3):286-96.
[3] Lin CT, Lin CR, Tan GK, et al. The mechanism of Epstein-Barr virus infection in nasopharyngeal carcinoma cells[J]. Am J Pathol,150(5): 1745-56.
[4] Niedobitek G. Epstein-Barr virus infection in the pathogenesis of nasopharyngeal carcinoma[J]. Mol Pathol, 53(5): 248-54.
[5] McClrue JE. Cellular receptor for Epstein-Barr virus[J]. Pro Med Virol, 1992, 39(2): 116-38.
[6] Lin CT, Kao HJ, Lin JL, et al. Response ofnasopharyngeal carcinoma cells to Epstein-Barr virus infecton in vitro[J]. Lab Invest,2000, 80(11): 1149-60.
[7] Bomsel M, Alfsen A. Entry of viruses through the epithelial barrier:pathogenic trickery[J]. Nat Rev Mol Cell Biol, 2003, 10(1): 57-68.
[8] 吕丽春,何英,蓝轲,等.人多聚免疫球蛋白受体转基因鼠的建立[J].第一军医大学学报,2003,23(2):127-9.Lu LC, He Y, Lan K, et al. Establishment of transgenic mouse models carrying human polymeric immunoglobulin receptor gene[J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2003, 23(2): 127-9.
[9] Hirumsatit R, Kongruttanachok N, Shotelersuk K, et al. Polymeric immunoglobulin receptor polymorphisms and risk of nasopharyngeal cancer[J]. BMC Gene, 2003, 4(1): 3-9.
[10] 任彩萍,刘卫东,何志巍,等.EBV感染及TPA处理前后人CR2转染细胞的基因差异表达谱[J].生物化学与生物物理学报,2001,33(1):105-11.Ren CP, Liu WD, He ZW, et al. Differentially expressed gene profiles of hCR2-transfected mouse cells before and after EBV infection and TPA treatment[J]. Acta Biochim Biophys Sin, 2001, 33(1):105-11.
[11] 江铁山,钟英丽.转录因子AP-2的研究进展[J].国外医学·分子生物学分册(Foreign Med·Mol Biol Sect),2003,25(1):21-4.
[12] Pepper MS, Ferrara N, Orci L, et al. Potent synergism between vascular endothelial growth factor and basic fibroblast growth factor in the induction ofangiogenesis in vitro[J]. Biochem Biochem Biophys Res Commun, 1992, 189(2): 824-31.
[13] 黄宗海,陈治,钱伟峰,等.血管内皮生长因子及其受体在大肠癌组织中的表达[J].第一军医大学学报,2001,2l(3):206-8.Huang ZH, Chen Z, Qian WF, et al. Expression of vascular endothelial growth factor and its receptor in colorectal carcinoma[J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2001, 21(3): 206-8.
[14] Rilok VP, Patricia G, Xie W, et al. Transforming growth factor-β signaling is disabled early in human endometrial carcinogenesis concomitant with loss of growth inhibition[J]. Cancer Res, 2002,62(32): 2778-90.
[15] Yasuyuki F, Minoru F, Norihiro M, et al. Reduced expression of transforming growth factor-β receptors is unfavorable prognostic factor in human esophageal squamous cell carcinoma[J]. Int J Cancer, 2003, 104(2): 161-6.
[16] Rebecca SM, Nacy D, Christoph AR, et al. Blockade of TGF-β inhibits mammary tumor cell viability, migration and metastases[J]. J Clin Invest, 2002, 109(16): 1551-9.
[17] Polakis P. Wnt signaling and cancer[J]. Genes Dev, 2000, 14(15):1837-51.