Journal of Southern Medical University ›› 2004, Vol. 24 ›› Issue (08): 925-927.

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MN/CAⅨ gene expression in renal cell carcinoma detected by RT-PCR

JIANG Yao-dong1, ZHENG Shao-bin1, WANG Zhan-hui2   

  1. 1. 第一军医大学南方医院泌尿外科, 广东, 广州, 510515;
    2. 第一军医大学南方医院感染内科实验室, 广东, 广州, 510515
  • Online:2004-08-20 Published:2004-08-20

Abstract: Objective To evaluate the reliability of reverse transcriptional (RT)-PCR in detecting MN/CAⅨ gene expression in renal cell carcinoma (RCC). Methods MN/CAⅨ gene expression was detected in 5 RCC tissues and 5 normal renal tissues. The primers of MN/CAⅨ gene were designed for a two-step RT-PCR, and the products examined by 2% agarose gel electrophoresis and identified by sequence analysis. Result Corresponding bands of MN/CAⅨ gene could be seen in RT-PCR products of cases RCC tissue from 4 but not in normal renal tissues. Conclusion RT-PCR is reliable for detecting the expression of MN/CAⅨgene in RCC.

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