Determination of dexamethasone sodium phosphate content in Fuyankang cream by high-performance capillary electrophoresis

Abstract

Abstract: Objective To determine the content of dexamethasone sodium phosphate in Fuyankang cream, a preparation for treatment of various skin conditions. Methods Dexamethasone sodium phosphate content in Fuyankang cream was determined using high-performance capillary electrophoresis (HPCE) at the detection wavelength of 254 nm, under the optimal condition achieved with a fused-silica capillary tube (60 cm×75 μm) and 100 mmol/L sodium tetraborate buffer (pH 9.2) at a constant voltage of 25 kV with a sampling time of 10 s at 25℃. Results The calibration curve displayed good linear relationship within dexamethasone sodium phosphate concentration range of 20 to 100μg/ml (r=0.999 8). The average recovery of dexamethasone sodium phosphate was 99.75% (n=5, RSD=1.03%). Conclusion HPCE is simple, quick and sensitive in determination of dexamethasone sodium phosphate content in Fuyankang cream.

CLC Number:

R927.2

GUO Dan¹, CHEN Na-na², YANG Xi-xiao¹, HOU Lian-bing¹. Determination of dexamethasone sodium phosphate content in Fuyankang cream by high-performance capillary electrophoresis[J]. Journal of Southern Medical University, 2004, 24(07): 839-840.

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