The first polar body of human oocytes does not help predict the precise location of the spindle

Abstract

Abstract: Objective To observe the precise position of the metaphase Ⅱ spindle for facilitating clinical practice of assisted reproduction. Methods LC-PolScope imaging system was used for observing the spindles in human metaphase Ⅱ oocytes matured in vitro or in vivo, and the angle between the center of the oocyte, the spindle and the first polar body was measured and compared between in vitro and in vivo matured oocytes. Results In vitro and in vivo matured oocytes were significantly different in the angle between the spindle and the first polar body (P=0.006). Conclusions The deviation of the spindle from the polar body in in vitro matured oocyte was significantly smaller than that in in vivo matured oocyte, and the first polar body does not help predict the precise location of the metaphase Ⅱ spindle. The imaging system is safe and effective for observing the spindles.

CLC Number:

R711

ZHU Liang, KONG Ling-hong, LI Hong, CHEN Shi-ling, XING Fu-qi. The first polar body of human oocytes does not help predict the precise location of the spindle[J]. Journal of Southern Medical University, 2004, 24(07): 809-811.

References

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