Dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during repair of muscle injury

Abstract

Abstract: Objective To observe the dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during the repair of injured muscle. Methods Muscular injury model was established by local injection of bupivacain, and at different time points following the injection, the gastrocnemius muscles were collected for preparation of cryosections. HE staining was performed for examination of the pathological changes in the injured muscles, and the expressions of MyoD and myogenin were detected by SABC. Results MyoD-positive nuclei began to appear 18 h after muscle injury, reaching the peak till 48 h after the injury. Myogenin-positive nuclei appeared 24 h after muscle injury and peaked at 72 h. Conclusions MyoD and myogenin play a role in muscle regeneration after muscle injury, and they may serve as the indexes to identify muscle precursor cells and mark muscle regeneration process.

CLC Number:

R746.2

ZENG Ying¹, ZHANG Cheng¹, LIU Ke-xuan², LI Cai-ming¹, FENG San-wei¹, LI Qun¹, LIU Tai-yun¹, HUANG Wen¹. Dynamic changes in the expressions of myogenic regulatory factors MyoD and myogenin during repair of muscle injury[J]. Journal of Southern Medical University, 2004, 24(05): 542-545.

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