Exon polymorphism of human RHD gene

Journal of Southern Medical University ›› 2004, Vol. 24 ›› Issue (05): 513-516.

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Exon polymorphism of human RHD gene

ZHOU Hua-you¹, LAN Jiong-cai¹, WANG Xiao-zhu², WANG Yi³, FAN Hong⁴, MENG Qing-bao⁵, GONG Hong-wei⁶

1. 第一军医大学南方医院输血科, 广东, 广州, 510515;
2. 秦皇岛市中心血站, 河北, 秦皇岛, 066000;
3. 河北省血液中心, 河北, 石家庄, 050000;
4. 锦州市红十字中心血站, 辽宁, 锦州, 121000;
5. 深圳市人民医院输血科, 广东, 深圳, 518000;
6. 江阴市中心血站, 江苏, 江阴, 214400

Online:2004-05-20 Published:2004-05-20

Abstract

Abstract: Objective To study exon polymorphism of human RHD gene and investigate the genetic mechanism of RhD-negative individuals. Methods PCR using sequence-specific primers (PCR-SSP) was performed on 40 RhD-positive, 120 RhD-negative and 2 weak D blood samples. Results All 10 exons could be detected in the 40 RhD-positive and 2 weak D samples. Out of the 120 RhD-negative samples, 28 (23.33%) carried 10 exons, 19 (15.83%) lost most of the 10 exons (with mainly intermediate deletion), and 73 (60.83%) had deletion of all the 10 exons; 19 samples of Del phenotype identified from the 120 RhD-negative samples had all the 10 exons. Conclusion Polymorphism of the exon structure of RHD gene is present in RhD-negative individuals, characterized chiefly by gross deletion, partial deletion and non-deletion.

CLC Number:

R446.11
R33

ZHOU Hua-you¹, LAN Jiong-cai¹, WANG Xiao-zhu², WANG Yi³, FAN Hong⁴, MENG Qing-bao⁵, GONG Hong-wei⁶. Exon polymorphism of human RHD gene[J]. Journal of Southern Medical University, 2004, 24(05): 513-516.

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Exon polymorphism of human RHD gene

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