Detection of serum Chlamydia pneumoniae(Cpn)immune complex and Cpn antibody in patients with coronary heart disease

Abstract

Abstract: Objective To understand the state of Chlamydia pneumoniae(Cpn) infection in patients with coronary heart dis-ease (CHD), and explore the relationship between Cpn infection and the gonesis and progressin of CHD. Method By adopt-ing sandwich method with double antibodies and indirect ELISA method, we detected serum Cpn immune complex and IgG antibody in 160 CHD patients (confirmed by coronary artery angiography) and 40 subjects without CHD. Results The posi-tivity rate of Cpn IgG type immune complex in the CHD group (63.8%) was significantly higher than that in the control group (40.0%, P<0.05), similar to the results of Cpn IgG antibody detection (65.0% vs32.5%, P<0.05). Conclusion Cpn infection is closely connected with the CHD, but their exact relationship needs further exploration and study.

CLC Number:

SUN Yun-yun¹, JIANG Wen-ling², LUO Xian-ling², CHEN Ji-yan¹. Detection of serum Chlamydia pneumoniae(Cpn)immune complex and Cpn antibody in patients with coronary heart disease[J]. Journal of Southern Medical University, 2004, 24(04): 365-366,391.

References

[1] Kuo CC, Shor A, Campbell LA, et al. Demonstration of Chlamydia pneumoniae in atherosclerotic lesions of coronary arteries[J]. J Infect Dis, 1993, 167(4):841-4.
[2] Kuo CC, Grayston JT, Campbell LA, et al. Chlamydia pneumoniae (TWAR)[J]. Clin Microbiol Rev, 1995, 8(4): 451-61.
[3] Gupta s, Leatham EW, Carrington D, et al. Elevated Chlamydia pneumoniae antibodies, cardiovascular events, and azithromycin in male survivors of myocardial infarction[J]. Circulation, 1997, 96(2): 404-7.
[4] Mendall MA, Carrington D, Strachan D, et al. Chlamydia pneumoniae: risk factors for seropositivity and association with coronary heart disease[J]. J Infect, 1995, 30 (2):121-8.
[5] Saikku P, Leinonen M, Mattila K, et al. Serological evidence of an association of a novel Chlamydia, TWAR, with chronic coronary heart disease and acute myocardial infection[J]. Lancet, 1988, 2(8618): 983-6.
[6] Yamashita K, Ouchi K, Shirai M, et al. Distribution of Chlamydia pneumoniae infection in the atherosclerotic carotic artery[J].Stroke, 1998, 29(4): 773-8.
[7] Davidson M, Kuo CC, Middaugh JP, et al. Confirmed previous infectiion with Chlamydia pneumoniae (TWAR) and its presence in early coronary atherosclerosis[J]. Circulation, 1998, 98(7): 628-33.
[8] Jackson LA, Campbell LA, Kno CC, et al. Isolation of Chlamydia pneumonia from a carotid atherosclerotic plaque specimen[J]. J Infect Dis, 1997,176(1): 292-5.
[9] Dahlen GH, Boman J. Brigander LS, et al. Lipoprotein, IgG, IgA and IgM antibodies to Chlamydia pneumoniae and HLA class Ⅱgenotype in early coronary artery disease[J]. Atheerosclerosis, 1995,114(1):165-74.
[10] Laurila A, Bloigu A, Nayha S, et al. Chronic Chlamydia pneumoniae infection is associated with a serum lipid profile known to be a risk factor for atherosclerosis[J]. Arterioscler Thromb Vasc Biol, 1997,17(11): 2910-3.
[11] Maass M, Bartels C, Engel PM, et al. Endovascular presence of viable Chlamydia pneumonioe is a common phenomenon in caronary artery disease[J]. J Am Coll Cardiol, 1998, 31 (4): 827-32.
[12] Maass M, Bartels C, Kruger S, et al. Endovascular presence of Chlamydia pneumoniae DNA is a generalized phenomenon in atherosclerotic vascular disease[J]. Atherosclerosis, 1998, 140(Supp Ⅱ): S25-S30.
[13] Sessa R, pietro MD, Santino I, et al. Chlamydia pneumoniae infection and atherosclerotic coronary disease[J]. Am Heart J, 1999,137(6): 1116-9.