Abstract: Objective To study how blood supply of the esophageal and gastric stumps influences the anastomotic healing after esophagogastrostomy in rabbits. Methods Twenty-seven New Zealand rabbits were randomly divided into 3 groups to receive esophagogastrostomy, followed by different procedures. Except for those in groupⅠ, all the rabbits were subjected to procedures of reducing the blood supply either of the esophageal or the gastric stump (group Ⅱ and group Ⅲ, respectively), followed by single-layer esophagogastric anastomoses using interrupted 5-0 polypropylene sutures. Ten days after operation, all the rats were killed and the anastomotic sites excised for measurement of the inner diameter, tensile strength, and hydroxyproline concentration. Results Healing of the esophagograstric anastomosis was obtained in all the rabbits but one with anastomotic leakage in groupⅠand one with perforation of the gastric fundus in groupⅢ. The anastomotic inner diameters were similar in all the three groups, whereas the tensile strength and hydroxyproline concentration at the anastomoses decreased in group Ⅲ in comparison with the other two groups (P<0.05) that had similar measurements (P>0.05). Conclusions Extended length of the free esophageal stump does not significantly affect anastomotic healing as decrease of blood supply in the gastric stump.