Abstract: Watershed algorithms is an automatic segmentation scheme to generate closed outlines, which might give rise to over-segmentation, i.e., numerous small segmented closed regions that blur the target contours or shapes. In this paper, the author proposed a scheme to merge the small regions and generate a hierarchical segmentation representation. We first define the dissimilarity measure between the neighboring regions, and based on this dissimilarity measurement, a fuzzy matrix calculation followed by hierarchical segmentation is performed. Experiments with various types of images are given.