Suppression of neutrophil immune function in rabbit models of hemorrhagic shock complicated by intra-abdominal hypertension

Abstract

Abstract: Objective To investigate the effects of hemorrhagic shock and intra-abdominal hypertension (IAH) on inflammatory responses of peripheral circulating neutrophils such as intracellular cytokine production, phagocytic capacity and expression of nuclear factor (NF)-κB. Methods Twenty-four rabbits were divided equally into 4 groups including a hemorrhagic shock (HS) group complicated by abdominal compartment syndrome (ACS) (Group A), a HS group (Group B), a ACS group (Group C) and a normal control group (Group D). Intracellular interleukin (IL)-8 production in the peripheral neutrophils were measured in the rabbits by flow cytometry, phagocytic function of the neutrophils evaluated by a chemiluminescence method and the NF-κB expression detected by immunocytochemistry before, immediately and 4 h after the traumatization. Results Four hours after the trauma, decreased intracellular IL-8 production and impaired phagocytic function of the peripheral neutrophils were observed in Group A along with suppressed NF-κB expression. But in Group B and Group C, the intracellular IL-8 production, phagocytic function and expression of NF-κB returned to the normal levels 4 hours after the trauma following the early-stage changes. In Group D, no significant changes occurred during the observation. Conclusions Responsiveness and function of the neutrophils to the stimuli by endotoxin are suppressed by the sequential second-hit of IAH after hemorrhagic shock, which may contribute to the occurrence of sepsis in ACS.

CLC Number:

R364.1

LU Qi¹, ZHUANG Yan-feng¹, WANG Wan-ming¹, CHENG Xue-ming¹, XU Hao¹, Yoshimasa Sakamoto², Sayoko Takahara², Masamichi Nishida², Kunio Kobayashi². Suppression of neutrophil immune function in rabbit models of hemorrhagic shock complicated by intra-abdominal hypertension[J]. Journal of Southern Medical University, 2004, 24(03): 325-328.

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