Detection of IgH gene rearrangement in Reed-Sternberg cells microdissected from classical Hodgkin’s lymphoma

Abstract

Abstract: Objective To study the origins and clonality of Hodgkin and Reed-Sternberg (H/RS) cells and their relations with the background lymphocytes in classical Hodgkin’s lymphoma. Method IgH gene rearrangement was detected in paffin-embedded tissues from 33 patients with Hodgkin’s lymphoma and a further analysis of the gene rearrangement was conducted in 6 of the positive cases identified after immunostaining of the sections with B-cell-specific activator protein (BSAP) followed by microdissection of the positivity labeled H/RS cells and background lymphocytes. Results IgH gene rearrangement was identified in 16 of the 33 cases. Microdissection of the lymphoma tissues was successfully performed in the 6 positive cases, and of the 19 tubes of H/RS cells obtained, 14 presented clonal bands of the rearrangement, and difference in cell numbers did not significantly influence the positive rate (P=0.290); in the 12 tubes of microdissected background lymphocytes obtained, 2 were positive for the rearrangement, and the positive rates for the rearrangement significantly differed between H/RS cells and the background lymphocytes (P=0.002). Conclusion The results appear to support the hypothesis that H/RS cells originates from B cells, and a part of the background lymphocytes may possess neoplastic proliferation potentials to function as the precursors of H/RS cells.

CLC Number:

R730.21

ZHOU Xinhua¹, ZHAO Tong¹, SHEN Xin-ming¹, YU Jiang², ZHU Mei-gang¹. Detection of IgH gene rearrangement in Reed-Sternberg cells microdissected from classical Hodgkin’s lymphoma[J]. Journal of Southern Medical University, 2004, 24(03): 286-289.

References

[1] Marafioti T, Hummel M, Foss HD, et al. Hodgkin and reed-stern berg cells represent an expansion of a single clone originating from a germinal center B-cell with functional immunoglobulin gene rear rangements but defective immunoglobulin transcription [J]. Blood, 2000, 95(4): 1443-50.
[2] 李甘地,邓飞.何杰金病H/R-S细胞起源和克隆性的研究进展(一)[J].诊断病理学杂志( J Diag Pathol),1999,6(1):43-4.
[3] Jaffe ES, Harris NL, Chan JK, et al. Proposed World Health Organi zation classification of neoplastic diseases of hematopoietic and lymphoid tissues[J]. Am J Surg Pathol, 1999, 93(3): 114-21.
[4] Scrideli CA, Defavery R, Bernardes JE, et al. Prognostic signifi cance of bi/oligoclonality in childhood acute lymphoblastic leukemia as determined by polymerase chain reaction [J]. Sao Paulo Med J, 2001, 119(5): 175-80.
[5] 张素娟,赵彤,董敬朋,等.石蜡切片DNA提取方法的改良及其临床应用[J].临床与实验病理学杂志(J Clin Exp Pathol),1996,12(4):363-4.
[6] 张素娟,余英豪.介绍一种新的免疫组化染色方法[J].第一军医大学学报(J FirstMilMedUniv/Di Yi Jun Yi Da Xue Xue Bao),1994,14(1):19.
[7] Hansmann ML, Kuppers R. Pathology and "molecular histology" of Hodgkin’s disease and the border to non-Hodgkin’s lymphomas [J]. Baillieres Clin Haematol, 1996, 9(3): 459-77.
[8] 江培洲,沈新明,黄华,等.显微操作仪快速分离癌细胞并提取微量RNA的方法[J].第一军医大学学报,2002,22(6):551-3.Jiang PZ, Shen XM, Huang H, et al. Rapid isolation of cancer from tumor tissue by micromanuiplator and extraction of tiny amount of RNA [J]. J First Mil Med Univ/Di Yi Jun Yi Da Xue Xue Bao, 2002, 22(6): 551-3.
[9] Uehira K, Amakawa R, Ito T, et al. A Hodgkin’s disease cell line, KM-H2, shows biphenotypic features of dendritic cells and B cells[J]. Int J Hematol, 2001, 73(2): 236-44.
[10] 周新华,赵彤,齐宗利,等.B细胞特异性激活蛋白在经典型霍奇金淋巴瘤中的表达及其意义[J].中华医学杂志,2002,82(22):1532-5.Zhou xinhua, Zhaotong, Qi zongli, et al. Expression and significa tion of B-cell-specific activator protein of H/RS cell in classical Hodgkin’s lymphoma[J]. Nat Med J Chin, 2002, 82(22): 1532-5.
[11] Foss HD, Reusch R, Demel G,et al. Frequent expression of the B cell-specific activator protein in Reed-Sternberg cells of classical Hodgkin’s disease provides further evidence for its B-cell origin [J]. Blood, 1999, 94(9): 3108-13.