Screening and identification of human telomerase reverse transcriptase epitopes from random phage display peptide library

Journal of Southern Medical University ›› 2004, Vol. 24 ›› Issue (03): 252-255.

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Screening and identification of human telomerase reverse transcriptase epitopes from random phage display peptide library

MIAO Pei-hong¹, LIU Bei-yi², ZHENG Shan-gen², PANG Jian-xin¹, XU Jiang-ping¹

1. 第一军医大学药理学教研室, 广东, 广州510515;
2. 第一军医大学免疫学教研室, 广东, 广州510515

Online:2004-03-20 Published:2004-03-20

Abstract

Abstract: Objective To identify and characterize the epitopes of human telomerase reverse transcriptase (hTERT). Methods A random phage displayed dodecapeptide library was screened with anti-hTERT antibody. The selected phage clones were identified by sandwich enzyme-linked immunosorbent assay, anti-hTERT antibody blocking assay and competitive inhibition assay. Results After 3 rounds of screening, 13 of 24 randomly selected phage clones were identified as positive clones that could specifically bind to anti-hTERT antibody but not to normal mouse IgG. Amino acid sequences deduced from DNA sequences showed 11 different sequences with high percentage of histone and hydrophilic amino acids. Conclusion Eleven epitopes have been obtained that can effectively mimic hTERT, which will be useful for the research of small-molecule inhibitor targeting at hTERT.

CLC Number:

MIAO Pei-hong¹, LIU Bei-yi², ZHENG Shan-gen², PANG Jian-xin¹, XU Jiang-ping¹. Screening and identification of human telomerase reverse transcriptase epitopes from random phage display peptide library[J]. Journal of Southern Medical University, 2004, 24(03): 252-255.

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Screening and identification of human telomerase reverse transcriptase epitopes from random phage display peptide library

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