Green tea polyphenols inhibit advanced glycation end product-induced rat vascular smooth muscle cell proliferation

Abstract

Abstract: Objective To determine the effects of green tea polyphenols(GTP) on advanced glycation end products (AGEs)-induced proliferation and expression of p44/42 mitogen-activated protein kinase (MAPK) of rat vascular smooth muscle cells (VSMCs). Methods Rat aortic VSMCs isolated and cultured in vitro were stimulated with AGEs in the presence or absence of GTP at different concentrations, followed by quantitative analysis of the cell proliferation with colorimetric assay. The p44/42 MAPK activity was evaluated by immunoblotting technique using anti-p44/42 phospho-MAPK antibody. Results Compared with the control cells(without GTP treatment), GTP dose-dependently inhibited AGE-stimulated VSMC proliferation (P<0.05), and the p44/42 MAPK activity was significantly enhanced. The effects of AGEs were antagonized by GTP (372±41 vs 761±56, P<0.05). Conclusion GTP can inhibit the AGE-induced proliferation and p44/42 MAPK expression of rat VSMCs.

OUYANG Ping¹, PENG Wen-lie², XU Ding-li¹, LAI Wen-yan¹, XU An-long². Green tea polyphenols inhibit advanced glycation end product-induced rat vascular smooth muscle cell proliferation[J]. Journal of Southern Medical University, 2004, 24(03): 247-251.

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