Detection of carbonic anhydrase Ⅸ gene expression in renal cell carcinoma with reverse transcription-PCR

Abstract

Abstract: Objective To evaluate the reliability of reverse transcription (RT) -PCR in detecting the expression of the carbonic anhydrase Ⅸ(MN/CAⅨ) gene in renal cell carcinoma (RCC).Methods MN/CAⅨwas examined in 5 cases of renal cell carcinoma tissues and 5 normal renal tissues. Primers specific for MN/CAⅨwas designed and two-step RT-PCR of the ex-tracted total RNA performed. The products was examined by 2% agrose electrophoresis and identified by sequence nanalysis.Results Specific bands of MN/CAⅨwere found in RT-PCR products of 4 case of RC C tissues, whereas could not be seen in normal renal tissues.Conclusion RT-PCR is reliable for detecting the expression of MN/CAⅨgene in RCC.

CLC Number:

R730.53

JIANG Yao-dong¹, ZHENG Shao-bin¹, WANG Zhan-hui². Detection of carbonic anhydrase Ⅸ gene expression in renal cell carcinoma with reverse transcription-PCR[J]. Journal of Southern Medical University, 2004, 24(01): 99-101.

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