Amplification and cloning of the N gene of SARS-associated coronavirus

Abstract

Abstract: Objective To amplify and clone the N g en e of severe acute respiratory syndrome-associated coronavirus.Method Using prim er Premier 5.0 software, two pairs of nested PCR primers were designed to amplif y the N gene. After purification, the amplified products were cloned into pMD18 -T vectors, and the positive clones with the inserted fragments were identified by sequence analysis.Results The amplified products was about 1 375 bp in leng th, and sequence analysis demonstrated that the N gene fragments had been succe ssfully inserted into pMD18-T vectors.Conclusion The successful amplification a nd cloning of N gene facilitates further investigation of the expression of the N protein and study of its structure and functions.

CLC Number:

Q786

XIAO Wei-wei¹, MA Wen-li¹, ZHANG Bao¹, WANG Yan¹, MAO Xiang-ming¹, PENG Yi-fei¹, SONG Yan-bin¹, WU Qing-hua¹, ZHENG Wen-ling². Amplification and cloning of the N gene of SARS-associated coronavirus[J]. Journal of Southern Medical University, 2004, 24(01): 39-41.

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