Abstract: Objective To conduct an In vitrostudy to ass es s the preliminary possibility of using formalin-fixed, instead of fresh, human bone tissues for allografting.Methods Fresh cadaveric bone tissues were fixed b y formalin for more than 6 months and dissected into 5mm×5 mm×5 mm pieces an d 5 mm×5 mm×40 mm sticks, followed by chemical treatments to prepare the allo graft bone materials. When alls treatments were completed, the bone grafts were centrifuged and their properties and cellular compatibility assessed in compari son with the currently used bone grafts clinically.Results The residual formal dehyde of the fixed allograft bone material was much below the controlled level and no significant differences were noted between the bone graft materials tes ted in regard to the chemical and mechanical properties and biocompatibility.Conclusion This material we have prepared may meet the clinical demands for bone grafting, with good biocompatibility and less chance for infection by pathologi cal agents.