Ussing chamber technique for studying the permeability of dexamethasone sodium phosphate liposome through rabbit colon mucosa In vitro

Abstract

Abstract: Objective To investigate the features of liposome-mediated drug permeability through the colon mucosaIn vitro.Methods Dexamethasone sodium phosphate liposome (DSP) was prepared using orthogonal design. With In vitroUs sing chamber experiment, the accumulated amount of DSP permeated through rabbit colon mucosa into the receptor chamber and the distribution amount in the colo n were determined at different time points during the experiment.Results Compar ed with the prepared DSP solution, DSP liposome decreased the permeated amount of DSP through rabbit colon mucosa. The apparent permeability coefficient (Papp,×10^-6,cm/s1) of the DSP was 31.95±7.65, significantly lower than that of DSP so lution (88.61±18.61). On the other hand, the distribution amount of DSP liposome in the colon mucosa was significantly higher than that of the DSP solution bo th at 120 and 300min.Conclusion DSP liposome dosage form may induce less toxic ity with better thera-peutic effect in the treatment of colitis, which is worthy of further exploitation.

CLC Number:

LI Guo-feng, CHEN Jia n-hai, YANG Jing, GUO Dan, REN Fei, WANG Chun-xia, HOU Lian-bin. Ussing chamber technique for studying the permeability of dexamethasone sodium phosphate liposome through rabbit colon mucosa In vitro[J]. Journal of Southern Medical University, 2004, 24(01): 11-14.

References

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